Potential aggregation hot spots in recombinant human keratinocyte growth factor: a computational study
نویسندگان
چکیده
منابع مشابه
cloning and expression of human keratinocyte growth factor in escherichia coli for recombinant drug production
results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...
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Nerve growth factor (NGF) is a neurotrophic factor that is functional in the survival, maintenance and differentiation of nervous system cells. This protein has three subunits, of which the beta subunit has the main activity. Its structure consists of a cysteine knot motif made up of beta strands linked by disulfide bonds. It can be used as a therapeutic agent in the treatment of many diseases....
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Keratinocyte growth factor (KGF) is considered to be one of the most important mitogens for lung epithelial cells. The objectives of this study were to confirm the effectiveness of intratracheal injection of recombinant human KGF (rhKGF) during compensatory lung growth and to optimize the instillation protocol. Here, trilobectomy in adult rat was performed, followed by intratracheal rhKGF insti...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Structure and Dynamics
سال: 2021
ISSN: 0739-1102,1538-0254
DOI: 10.1080/07391102.2021.1908912